Research Technologist 1- Morimoto Lab- Northwestern University
Conducts tests and procedures; sets up and operates equipment; records observations and measurements and collects and compiles data in a research lab; requires a practical understanding of a limited number of standard scientific techniques.
The Morimoto laboratory at Northwestern University, Department of Molecular Biosciences, Evanston, IL is seeking a highly qualified post-graduate undergraduate student to join our research team in a Research Technologist I position (minimum 2 year desired). Our lab uses mammalian cell culture assays to study proteostasis taking multidisciplinary approaches including molecular biology, genetics, biochemistry, and chemical biology to explore the mechanisms involved in neurodegenerative disease. This position includes utilizing mammalian cell culture for reprogramming human fibroblasts into neurons. The task may require a significant time investment, and it is essential for the candidate to demonstrate accountability in its execution.
This unique opportunity would involve the candidate working closely with a postdoctoral fellow to carry forward research that would contribute toward both presentations and publications. We are looking for someone who could commit to a minimum of 2 years and are considering applying to graduate school programs. Experience with cell culture is highly preferred. The candidate should also have advanced experience in molecular biology, protein biochemistry, structural biology, genetics and/or genomics-centered methodologies, through prior lab research experience as well as undergraduate coursework. The ideal candidate will be highly motivated, goal-oriented, able to work both independently and in teams, and will possess excellent organizational, communication, and analytical skills. Applicant must be competent to carefully follow instructions, extremely reliable and able to conduct and troubleshoot experiments, keep experimental records and analyze data.
Preferred qualifications: experience with mammalian cell culture, molecular biological techniques such as electrophoresis (DNA gel and SDS PAGE), Western, and PCR. Able to prepare and maintain laboratory stock reagents and supplies and maintain excellent organizational skills.
This will be a 1-year renewable position based upon performance and funding availability. This position is a FULL-TIME position at 40.0 hours per week and works on-site.
Target hiring range for this position will be between $16.55 – $23.28 per hour. Offered salary will be determined by the applicant’s education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data.
Specific Responsibilities:
- Follows pre-defined protocols, policies, procedure, regulations, Good Clinical and Good Laboratory Practices and conducts standard and repetitive experiments and completes basic trouble-shooting techniques.
- Sets-up, operates and maintains standard lab equipment.
- Molecular Biology:
- DNA, RNA, Protein isolation, purification and expression
- Southern, Northern, Western blotting
- Cloning, mapping and sequencing of DNA
- Recombinant virus production and purification
- Functional studies
- Histology:
- Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining
- Immunohistochemistry
- Other (please specify):
- Buffer, reagent and media preparation
- Microscopy
- Records test results and may maintain databases.
- Completes standard and routine clerical duties such as copying, filing, data entry, word processing, etc.
- Maintains inventory of reagents, mice and lab supplies.
- Completes other duties as assigned.
Minimum Qualifications:
- HS diploma or equivalent and some basic science courses is required.
Minimum Competencies:
- Advanced experience in molecular biology, protein biochemistry, structural biology, genetics, and/or genomics—centered methodologies, through prior lab research experience as well as undergraduate coursework.
- Highly motivated, goal oriented.
- Able to multi-task and prioritize responsibilities.
- Able to work both independently and in teams.
- Possess excellent organizational and communication skills.
- Possess excellent analytical skills.
- Ability to read/comprehend scientific literature.
- Can be adaptable and willing to learn.
Preferred Qualifications:
- Bachelors degree in Biology, Neurobiology, Molecular Biology or related field.
Preferred Competencies:
- Experience with mammalian cell culture.
- Experience with molecular biological techniques such as electrophoresis (DNA gel and SDS PAGE), Western, and PCR.
- Able to prepare and maintain laboratory stock reagents and supplies.
- Have excellent organization skills and competency in maintaining a detailed laboratory notebook.
- Experience with mammalian cell neuronal conversion or lentivirus systems.
- Competent to follow protocols, plan, prepare, execute and troubleshoot experiments.
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