About Affini-T Therapeutics
Affini-T Therapeutics is a clinical stage biotechnology company focused on building a “best-in-class” TCR therapy platform. Our innovative method of discovering high affinity TCRs specific to oncogenic driver mutations is used to develop solid tumor therapies. Through the application of gene editing and synthetic biology, we focus on enhanced T cell fitness to overcome the immunosuppressive tumor microenvironment. Job Summary
We are seeking a highly motivated bench Scientist to support and develop Affini-T’s proprietary TETHERTM platform for generating highly potent and specific T-cell engagers for solid tumors. The Scientist will have the opportunity to play a critical role in impacting all aspects of the platform including TCR affinity maturation, protein engineering and production, and characterization of T cell engager molecules.
Essential Duties and Responsibilities
The major tasks for this position are as follows:- Design and generate expression constructs for the expression of T cell engagers from mammalian systems
- Express, purify, and characterize novel T cell engagers to support screening and lead optimization in discovery programs
- Construct high diversity libraries and execute TCR affinity maturation campaigns
- Maintain accurate records of inventory, experiments, protocols, and data analysis for documentation and reporting purposes
- Participate in the preparation and delivery of scientific presentations for team meetings
Desired and Required Qualifications
Education and Experience: - PhD in Biochemistry, Biology, or related field, with 1+ year of industry experience, MS with 5+ years of industry experience, or BS with 8+ years of industry experience
- Demonstrated experience with mammalian cell culture required
- Experience with expression construct design and molecular biology (including cloning, qPCR, and next-generation sequencing)
- Experience with assay development to assess immune cell engagers including ELISA, tumor cell killing, cytokine secretion, multi-parameter flow cytometry, and T cell activation assays
- Experience in purification of proteins with affinity, ion exchange, size exclusion, hydrophobic interaction chromatography preferred
- Experience with AKTA FPLC systems for protein purification preferred
- Ability to work well as both team member and as individual contributor
- Excellent communication skills for cross-functional, management and external interactions; excellent oral presentation skills are critical
- Strong organizational skills to ensure thorough and timely documentation of experiments, and preparation of data for compiling reports and presentations to research and corporate teams
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